![]() ProtCID - Protein common interface database (for homologous proteins).Proteopedia: essentially a wikipedia for proteins.RCSB (Research Collaboratory for Structural Bioinformatics, US).literature-based curation of the K12-MG1655 genome.BsaI restriction sites will be removed no (BsaIsite) constraint. Flanking promoter and terminator regions are protected against changes by keep annotations (shorthand for AvoidChanges). EcoGene (when it was live it was great) Gene NUM1 of Saccharomyces cerevisiae will be codon optimized for Escherichia coli, and have a GC content of 4060 on every 100-nucleotide window.list of strain genotypes and what the genotypes mean.Commandments of experimental microbiology (Barrick).They often have stock primers for standard plasmids. Follow instructions on provider’s webpage about how to prepare samples.Electroporation - see wet-lab-protocols.Type IIS cloning combined with PCR cloning (= recommended as default).Traditional cloning (Type II restriction digest).Enzymes used in molecular biology: a useful guide.Snapgene has Tm calculations that work OK.Andersen promoters (set of constititive promoters from weak to strong): iGEM pages.Restriction enzymes - SnapGene, EnzymeX, NEB website.Though Cam is often used at 34µg/ml final, slightly more than 25 µg/ml. Antibiotic resistance genes - Vector/plasmid database.iGEM Registry databases and distributions. We also recommend SnapGene Viewer as a helpful.Snapgene (£££) - DNA analysis package.Benchling (free) - Labbook and DNA analysis package.This paper is great resource not just for Tricine gels but also for hydrophobic proteins, membrane proteins, SDS, DTT/beta-merc reducing agents, etc. Tricine-SDS-PAGE for small proteins and peptides Kits: Minipreps, PCR purification, Gel extraction - manuals within the boxes.Reagent-specific info can be found on the manufacturer’s website.Clustal Omega, DNA/RNA/Protein alignment.Note that this list isn’t meant to be exhaustive, it is mainly to get you started. coli, and to help you to begin to troubleshoot your own experiments. Here are a list of online resources that should help you find information about DNA part sequences, molecular biology questions or basic protocols for handling DNA/RNA/proteins and E. Nowadays there are so many resources online to help you with all basic protocols, but it helps to know where to start. As a result, each gene optimization is fully customized to maximize the chance of obtaining a functional and active protein. ![]() This list of resources was primarily created to help students new to the wet lab, or to molecular and microbiological techniques. Will be updated/overhauled over the coming months.
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